In silico Analysis of Pasteurella multocida PlpE Protein Epitopes As Novel Subunit Vaccine Candidates

Authors

  • Abbas Ghasemzadeh Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
  • Gholamreza Nikbakht Brujeni Department of Microbiology and Immunology, University of Tehran, Iran
  • Mohammad Ali Shokrgozar National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  • Mohsen Abolhassani Hybridoma Lab, Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
  • Parastoo Ehsani Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
  • Saied Mostaan Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
  • Soroush Sardari Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Abstract:

Background: Pasteurella multocida is a Gram-negative, non-motile, non-spore forming, and aerobic/anaerobic cocobacillus known as the causative agent of human and animal diseases. Humans can often be affected by cat scratch or bite, which may lead to soft tissue infections and in rare cases to bacteremia and septicemia. Commercial vaccines against this agent include inactivated, live attenuated, and non-pathogenic bacteria. Current vaccines have certain disadvantages such as reactogenicity or reversion to virulence. Therefore, the aim of this study was to reach a multi-epitope vaccine candidate that could be serotype independent and covers most incident serotypes of P. multocida. Methods: In this study, reverse vaccinology strategy was used to identify potentially immunogenic and protective epitopes. First, multiple alignments of different sequences of Pasteurella lipoprotein E (PlpE) from various serotypes of P. multocida were analyzed to identify the conserved regions. Bioinformatics tools were then applied to predict and select epitopes for further studies. Results: Three different conserved immunogenic regions were selected according to the selected criteria, and their various sequential orders were evaluated structurally by in silico tools to find the best order. Conclusion: In searching the epitopes of PlpE to design a new vaccine candidate against pasteurellosis, we found the region 1 + region 2 + region 3 (without any linker between regions) of epitope, including the regions of PlpE protein of P. multocida, as the appropriate serotype independent vaccine candidate against pasteurellosis.

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Journal title

volume 25  issue 1

pages  41- 46

publication date 2021-01

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